Genetic characterization and protective immunity of cold-adapted attenuated avian H9N2 influenza vaccine
Identifieur interne : 001346 ( Main/Exploration ); précédent : 001345; suivant : 001347Genetic characterization and protective immunity of cold-adapted attenuated avian H9N2 influenza vaccine
Auteurs : JOO SUB LEE [Corée du Sud] ; HYUN SOO KIM [Corée du Sud] ; SANG HEUI SEO [Corée du Sud]Source :
- Vaccine [ 0264-410X ] ; 2008.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
Abstract
H9N2 influenza viruses are endemic in many Asian countries including China and Korea, and cause a considerable economic loss to chicken industry by reduction in egg production and about 30% mortality. Here we developed live cold-adapted attenuated H9N2 influenza vaccine by adaptation of viruses in hen's eggs at 25 °C. Genetic analysis shows that the cold-adapted H9N2 (A/Chicken/Korea/Sl/03) viruses contain a total of 44 amino acid substitutions, of which 7 amino acids are identical to the loci identified in the cold-adapted H2N2 (A/Ann Arbor/6/60) vaccine strain compared to genes in wild-type H9N2 (A/Chicken/Korea/S1/03) influenza viruses. When cold-adapted H9N2 (A/Chicken/Korea/S1/03) influenza viruses were inoculated in layers viruses were detectable in the tracheas, not in the lungs, no reduction of egg production and mortality was observed in contrast to the infection of wild-type H9N2 influenza viruses, and CD8+ T lymphocytes expressing IFN-γ were induced. When layers vaccinated with cold-adapted attenuated H9N2 (A/Chicken/Korea/S1/03) influenza viruses were challenged with wild-type H9N2 (A/Chicken/Korea/S21/04) influenza viruses, they were protected from the loss of egg production and mortality. Our results suggest that cold-adapted attenuated H9N2 vaccine can be used for controlling the infection of H9N2 influenza viruses in chickens.
Affiliations:
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xml:lang="fr"><term>Génétique</term><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H9N2 influenza viruses are endemic in many Asian countries including China and Korea, and cause a considerable economic loss to chicken industry by reduction in egg production and about 30% mortality. Here we developed live cold-adapted attenuated H9N2 influenza vaccine by adaptation of viruses in hen's eggs at 25 °C. Genetic analysis shows that the cold-adapted H9N2 (A/Chicken/Korea/Sl/03) viruses contain a total of 44 amino acid substitutions, of which 7 amino acids are identical to the loci identified in the cold-adapted H2N2 (A/Ann Arbor/6/60) vaccine strain compared to genes in wild-type H9N2 (A/Chicken/Korea/S1/03) influenza viruses. When cold-adapted H9N2 (A/Chicken/Korea/S1/03) influenza viruses were inoculated in layers viruses were detectable in the tracheas, not in the lungs, no reduction of egg production and mortality was observed in contrast to the infection of wild-type H9N2 influenza viruses, and CD8+ T lymphocytes expressing IFN-γ were induced. When layers vaccinated with cold-adapted attenuated H9N2 (A/Chicken/Korea/S1/03) influenza viruses were challenged with wild-type H9N2 (A/Chicken/Korea/S21/04) influenza viruses, they were protected from the loss of egg production and mortality. Our results suggest that cold-adapted attenuated H9N2 vaccine can be used for controlling the infection of H9N2 influenza viruses in chickens.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Joo Sub Lee" sort="Joo Sub Lee" uniqKey="Joo Sub Lee" last="Joo Sub Lee">JOO SUB LEE</name></noRegion><name sortKey="Hyun Soo Kim" sort="Hyun Soo Kim" uniqKey="Hyun Soo Kim" last="Hyun Soo Kim">HYUN SOO KIM</name><name sortKey="Sang Heui Seo" sort="Sang Heui Seo" uniqKey="Sang Heui Seo" last="Sang Heui Seo">SANG HEUI SEO</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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